Impeller types and feeding modes influence the morphology and protein expression in the submerged culture of Aspergillus oryzae

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Title
Impeller types and feeding modes influence the morphology and protein expression in the submerged culture of Aspergillus oryzae
Author(s)
Joo-Hyung Heo; V Ananin; Jeong Seok Park; Chung Ryul Lee; J O Moon; Oh Suk Kwon; Hyun Ah Kang; Chul Ho Kim; Sang Ki Rhee
Bibliographic Citation
Biotechnology and Bioprocess Engineering, vol. 9, no. 3, pp. 184-190
Publication Year
2004
Abstract
The influences of impeller types on morphology and protein expression were investigated in a submerged culture of Aspergillus oryzae. The impeller types strongly affected mycelial morphology and protein production in batch and fed-batch fermentations. Cells that were cultured by propeller agitation grew in the form of a pellet, whereas cells that were cultured by turbine agitation grew in a freely dispersed-hyphal manner and in a clumped form. Pellet-grown cells showed high levels of protein production for both the intracellularly heterologous protein (β-glucuronidase) and the extracellularly homologous protein (α-amylase). The feeding mode of the carbon source also influenced the morphological distribution and protein expression in fed-batch fermentation of A. oryzae. Pulsed-feeding mainly showed high protein expression and homogeneous distribution of pellet whereas continuous feeding resulted in less protein expression and heterogeneous distribution with pellet and dispersed-hyphae. The pellet growth with propeller agitation paralleling with the pulsed-feeding of carbon source showed a high level of protein production in the submerged fed-batch fermentation of recombinant A. oryzae.
Keyword
aspergillus oryzaefed-batch fermentationfilamentous fungimorphologypropeller agitationsubmerged culture
ISSN
1226-8372
Publisher
South Korea
DOI
http://dx.doi.org/10.1007/BF02942290
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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