Effect of Astilbe koreana on ultraviolet B (UVB)-induced inflammatory response in human keratinocytes

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Title
Effect of Astilbe koreana on ultraviolet B (UVB)-induced inflammatory response in human keratinocytes
Author(s)
M K Na; Byung Sun Min; Ren Bo An; K S Song; Y H Seong; K H Bae
Bibliographic Citation
Biological & Pharmaceutical Bulletin, vol. 27, no. 8, pp. 1301-1304
Publication Year
2004
Abstract
The EtOH extract from the rhizomes of Astilbe koreana (Saxifragaceae) exhibited potent antioxidant activity in our recent study. Since the oxidative stress is known to be involved in the inflammatory response after ultraviolet B (UVB) exposure, the ability of the A. koreana extract to inhibit UVB-induced prostaglandin E2 (PGE2) and nitric oxide (NO) production was examined. UVB irradiation (35 mJ/cm2) increased PGE2 and NO production, which were significantly decreased by pre-administration of the A. koreana extract in a dose-dependent manner. The A. koreana extract also preserved cellular antioxidant capacity after UVB irradiation, which was determined by glutathione (GSH) content. UVB irradiation enhanced the formation of ROS in the keratinocytes, which was determined using a 2,7′- dichlorofluorescein diacetate (DCFH-DA), a redox sensitive dye. The levels of intracellular reactive oxygen species (ROS) were also significantly reduced by pre-treatment of the A. koreana extract in a dose-dependent manner measured 9 h after UVB irradiation. The results suggest that the A. koreana extract may have a protective effect on the UVB-injured keratinocytes by inhibiting PGE 2 and NO production, possibly through the inhibition of intracellular ROS accumulation.
Keyword
Astilbe koreanaIntracellular reactive oxygen species (ROS)SaxifragaceaeUltraviolet B (UVB)-induced prostaglandin E2 (PGE2) productionUVB-induced nitric oxide (NO) production
ISSN
0918-6158
Publisher
Pharmaceutical Soc Japan
DOI
http://dx.doi.org/10.1248/bpb.27.1301
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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