Development of an Agrobacterium-mediated transient expression system for intact leaves of chill pepper = Agrobacterium을 이용한 고추의 transient expression 시스템

Cited 0 time in scopus
Metadata Downloads
Title
Development of an Agrobacterium-mediated transient expression system for intact leaves of chill pepper = Agrobacterium을 이용한 고추의 transient expression 시스템
Author(s)
Eun Soo Seong; Young Hee Joung; Doil Choi
Bibliographic Citation
Korean Journal of Plant Biotechnology (Tissue Culture), vol. 31, no. 3, pp. 185-190
Publication Year
2004
Abstract
We established a transient gene expression system in chili pepper leaves based on Agrobacterium-mediated transformation of GUS gene. For the best GUS transient expression, two step culture system was adopted. When the Agrobacterium tumefaciens cell density of pre-culture was A∧600nm 0.3, the cells were harvested and diluted to A∧600nm 0.8 with virulence induction medium after cell harvested. The addition of acetosyringone (200 μM) in virulence induction step was a key factor for successful transient expression.
Keyword
acetosyringone (AS)agrobacterium-mediated transformationchili peppertransient expression
ISSN
1015-5880
Publisher
South Korea
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.