DC Field | Value | Language |
---|---|---|
dc.contributor.author | Eun Soo Seong | - |
dc.contributor.author | Young Hee Joung | - |
dc.contributor.author | Doil Choi | - |
dc.date.accessioned | 2017-04-19T09:01:44Z | - |
dc.date.available | 2017-04-19T09:01:44Z | - |
dc.date.issued | 2004 | - |
dc.identifier.issn | 1015-5880 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/6685 | - |
dc.description.abstract | We established a transient gene expression system in chili pepper leaves based on Agrobacterium-mediated transformation of GUS gene. For the best GUS transient expression, two step culture system was adopted. When the Agrobacterium tumefaciens cell density of pre-culture was A∧600nm 0.3, the cells were harvested and diluted to A∧600nm 0.8 with virulence induction medium after cell harvested. The addition of acetosyringone (200 μM) in virulence induction step was a key factor for successful transient expression. | - |
dc.publisher | Korea Soc-Assoc-Inst | - |
dc.title | Development of an Agrobacterium-mediated transient expression system for intact leaves of chill pepper = Agrobacterium을 이용한 고추의 transient expression 시스템 | - |
dc.title.alternative | Development of an Agrobacterium-mediated transient expression system for intact leaves of chill pepper | - |
dc.type | Article | - |
dc.citation.title | Korean Journal of Plant Biotechnology (Tissue Culture) | - |
dc.citation.number | 3 | - |
dc.citation.endPage | 190 | - |
dc.citation.startPage | 185 | - |
dc.citation.volume | 31 | - |
dc.contributor.affiliatedAuthor | Eun Soo Seong | - |
dc.contributor.affiliatedAuthor | Young Hee Joung | - |
dc.contributor.affiliatedAuthor | Doil Choi | - |
dc.contributor.alternativeName | 성은수 | - |
dc.contributor.alternativeName | 정영희 | - |
dc.contributor.alternativeName | 최도일 | - |
dc.identifier.bibliographicCitation | Korean Journal of Plant Biotechnology (Tissue Culture), vol. 31, no. 3, pp. 185-190 | - |
dc.subject.keyword | acetosyringone (AS) | - |
dc.subject.keyword | agrobacterium-mediated transformation | - |
dc.subject.keyword | chili pepper | - |
dc.subject.keyword | transient expression | - |
dc.subject.local | acetosyringone (AS) | - |
dc.subject.local | Agrobacterium-mediated transformation | - |
dc.subject.local | agrobacterium-mediated transformation | - |
dc.subject.local | Chili pepper (Capsicum annuum) | - |
dc.subject.local | Chili pepper | - |
dc.subject.local | Chili pepper (Capsicum annum) | - |
dc.subject.local | chili pepper | - |
dc.subject.local | transient expression | - |
dc.subject.local | Transient expression | - |
dc.description.journalClass | N | - |
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