Development of an Agrobacterium-mediated transient expression system for intact leaves of chill pepper = Agrobacterium을 이용한 고추의 transient expression 시스템

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dc.contributor.authorEun Soo Seong-
dc.contributor.authorYoung Hee Joung-
dc.contributor.authorDoil Choi-
dc.date.accessioned2017-04-19T09:01:44Z-
dc.date.available2017-04-19T09:01:44Z-
dc.date.issued2004-
dc.identifier.issn10155880-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6685-
dc.description.abstractWe established a transient gene expression system in chili pepper leaves based on Agrobacterium-mediated transformation of GUS gene. For the best GUS transient expression, two step culture system was adopted. When the Agrobacterium tumefaciens cell density of pre-culture was A∧600nm 0.3, the cells were harvested and diluted to A∧600nm 0.8 with virulence induction medium after cell harvested. The addition of acetosyringone (200 μM) in virulence induction step was a key factor for successful transient expression.-
dc.publisherSouth Korea-
dc.titleDevelopment of an Agrobacterium-mediated transient expression system for intact leaves of chill pepper = Agrobacterium을 이용한 고추의 transient expression 시스템-
dc.title.alternativeDevelopment of an Agrobacterium-mediated transient expression system for intact leaves of chill pepper-
dc.typeArticle-
dc.citation.titleKorean Journal of Plant Biotechnology (Tissue Culture)-
dc.citation.number3-
dc.citation.endPage190-
dc.citation.startPage185-
dc.citation.volume31-
dc.contributor.alternativeName성은수-
dc.contributor.alternativeName정영희-
dc.contributor.alternativeName최도일-
dc.identifier.bibliographicCitationKorean Journal of Plant Biotechnology (Tissue Culture), vol. 31, no. 3, pp. 185-190-
dc.subject.keywordacetosyringone (AS)-
dc.subject.keywordagrobacterium-mediated transformation-
dc.subject.keywordchili pepper-
dc.subject.keywordtransient expression-
dc.subject.localacetosyringone (AS)-
dc.subject.localagrobacterium-mediated transformation-
dc.subject.localAgrobacterium-mediated transformation-
dc.subject.localchili pepper-
dc.subject.localChili pepper-
dc.subject.localtransient expression-
dc.subject.localTransient expression-
dc.description.journalClassN-
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