Development of an Agrobacterium-mediated transient expression system for intact leaves of chill pepper = Agrobacterium을 이용한 고추의 transient expression 시스템

Abstract

We established a transient gene expression system in chili pepper leaves based on Agrobacterium-mediated transformation of GUS gene. For the best GUS transient expression, two step culture system was adopted. When the Agrobacterium tumefaciens cell density of pre-culture was A∧600nm 0.3, the cells were harvested and diluted to A∧600nm 0.8 with virulence induction medium after cell harvested. The addition of acetosyringone (200 μM) in virulence induction step was a key factor for successful transient expression.