Bax-induced cell death of Arabidopsis is meditated through reactive oxygen-dependent and -independent processes

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Title
Bax-induced cell death of Arabidopsis is meditated through reactive oxygen-dependent and -independent processes
Author(s)
D Baek; J Nam; Y D Koo; D H Kim; Jiyoung LeeJae Cheol JeongSang Soo Kwak; W S Chung; C O Lim; J D Bahk; J C Hong; S Y Lee; M Kawai-Yamada; H Uchimiya; D J Yun
Bibliographic Citation
Plant Molecular Biology, vol. 56, no. 1, pp. 15-27
Publication Year
2004
Abstract
An Arabidopsis protoplast system was developed for dissecting plant cell death in individual cells. Bax, a mammalian pro-apoptotic member of the Bcl-2 family, induces apoptotic-like cell death in Arabidopsis. Bax accumulation in Arabidopsis mesophyll protoplasts expressing murine Bax cDNA from a glucocorticoid-inducible promoter results in cytological characteristics of apoptosis, namely DNA fragmentation, increased vacuolation, and loss of plasma membrane integrity. In vivo targeting analysis monitored using jellyfish green fluorescent protein (GFP) reporter indicated full-length Bax was localized to the mitochondria, as it does in animal cells. Deletion of the carboxyl-terminal transmembrane domain of Bax completely abolished targeting to mitochondria. Bax expression was followed by reactive oxygen species (ROS) accumulation. Treatment of protoplasts with the antioxidant N-acetyl-L-cysteine (NAC) during induction of Bax expression strongly suppressed Bax-mediated ROS production and the cell death phenotype. However, some population of the ROS depleted cells still induced cell death, indicating that there is a process that Bax-mediated plant cell death is independent of ROS accumulation. Accordingly, suppression of Bax-mediated plant cell death also takes place in two different processes. Over-expression of a key redox-regulator, Arabidopsis nucleoside diphosphate kinase 2 (AtNDPK2) down-regulated ROS accumulation and suppressed Bax-mediated cell death and transient expression of Arabidopsis Bax inhibitor-1 (AtBI-1) substantially suppressed Bax-induced cell death without altering cellular ROS level. Taken together, our results collectively suggest that the Bax-mediated cell death and its suppression in plants is mediated by ROS-dependent and -independent processes.
Keyword
ApoptosisArabidopsis protoplastsBaxCell deathMitochondriaReactive oxygen species
ISSN
0167-4412
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s11103-004-3096-4
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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