Manufacturing protein-DNA chip for depigmenting agent screening = 전사인자 저해제 통한 미백제 탐색용 단백질 칩 제작

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Title
Manufacturing protein-DNA chip for depigmenting agent screening = 전사인자 저해제 통한 미백제 탐색용 단백질 칩 제작
Author(s)
J S Han; E Y Kwak; H B Lee; J H Shin; Seung Hak Baek; Bong Hyun Chung; E K Kim
Bibliographic Citation
Journal of Society of Cosmetic Scientists of Korea, vol. 30, no. 4, pp. 479-483
Publication Year
2004
Abstract
An attempt was made to develop a proteinchip for screening of MITF (microphthalmia transcription factor) inhibitor. Binding of MITF to E-box causes transcription of several pigmenting genes including tyrosinase gene. We investigated binding of MITF and its DNA binding site (E-box) using a protein-DNA chip with various detection methods including flurorescence (Cyt3). SPR (surface plasmon resonance) and SPRi (surface plasmon resonance imaging). A fusion protein (MITF-Maltose Binding Protein) was attached on the glass plate by chemical modification. An inhibitory synthetic DNA oligomer, artificially designed based on the E-box sequence, inhibited the binding of MITF and E-box. These results showed the potentials of flurorescence-based MITF protein chip as a microarray for high throughput screening (HTS) system of depigmenting agents.
Keyword
MITF(microphthalmia transcription factor)E-boxβ-CDmicroarrayfluoresence-based detection
ISSN
1226-2587
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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