Molecular cloning of levan fructotransferase gene from Arthrobacter ureafaciens K2032 and its expression in Escherichia coli for the production of difructose dianhydride IV

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Title
Molecular cloning of levan fructotransferase gene from Arthrobacter ureafaciens K2032 and its expression in Escherichia coli for the production of difructose dianhydride IV
Author(s)
Chul Ho Kim; E K Jang; S H Kim; K H Jang; S A Kang; K B Song; Oh Suk Kwon; Sang Ki Rhee
Bibliographic Citation
Letters in Applied Microbiology, vol. 40, no. 3, pp. 228-234
Publication Year
2005
Abstract
Aims: To clone and overexpress a novel levan fructotransferase gene lftA from Arthrobacter ureafaciens K2032. Methods and Results: The lftA gene, encoding a levan fructotransferase (LFTase) of 521 amino acids (aa) residues, was cloned from the genomic DNA of A. ureafaciens K2032, and overexpressed in Escherichia coli. The recombinant LFTase overexpressed in E. coli was then used to produce a difructose dianhydride (DFA IV) from levan. DFA IV crystals with 97% purity could be obtained from the reaction mixture in 83-7% yield by using a natural crystallization method. Conclusions: The lftA gene cloned from A. ureafaciens K2032 encode a novel levan fructotransferase which produces difructose dianhydride (DFA IV) from levan. Significance and Impact of the Study: Levan fructotransferase is a useful enzyme with great promise in the production of DFA IV and various fructosides.
Keyword
Absorption factor of mineralsDFAFructosyltransferaseLow-calorie sweetener
ISSN
0266-8254
Publisher
Wiley
DOI
http://dx.doi.org/10.1111/j.1472-765X.2005.01658.x
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
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