Identification of recombinant subtilisins

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Title
Identification of recombinant subtilisins
Author(s)
Nack Shick Choi; Ki Hyun Yoo; Kab Seog Yoon; Kyu Tae Chang; P J Maeng; Seung Ho Kim
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 15, no. 1, pp. 35-39
Publication Year
2005
Abstract
To identify the activity of recombinant subtilisins (subtilisin BPN' and subtilisin Carlsberg), three different zymography methods, SDS-fibrin zymography (SDS-FZ), reverse fibrin zymography (RFZ), and isoelectric focusing-fibrin zymography (IEF-FZ), were used. The recombinant subtilisins BPN' and Carlsberg did not migrate into the electrophoretic field based on a Laetymili buffer system, instead forming a "binding mode" at the top part of the separating gels with the SDS-FZ and RFZ techniques. Yet, this problem was resolved when using IEF-FZ with a pH range from 3 to 10. In addition, all these methods enabled the activity of a recombinant pro-subtilisin DJ-4 to be detected without a refolding pathway.
Keyword
bacillusbinding modefibrin zymographyisoelectric focusingrecombinant subtilisin
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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