Open Access@KRIBBDivision of Research on National ChallengesBionanotechnology Research Center1. Journal Articles
Enhanced sensitivity of surface plasmon resonance (SPR) immunoassays using a peroxidase-catalyzed precipitation reaction and its application to a protein microarray
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- Title
- Enhanced sensitivity of surface plasmon resonance (SPR) immunoassays using a peroxidase-catalyzed precipitation reaction and its application to a protein microarray
- Author(s)
- Min-Gon Kim; Yong Beom Shin; Jin Mi Jung; H S Ro; Bong Hyun Chung
- Bibliographic Citation
- Journal of Immunological Methods, vol. 297, no. 1, pp. 125-132
- Publication Year
- 2005
- Abstract
- We describe a method to improve the sensitivity of surface plasmon resonance (SPR) immunoassays using a horseradish peroxidase (HRP)-catalyzed precipitation reaction. The precipitation reaction catalyzed by HRP bound to the SPR biosensor surface via a sandwich immunoassay induced a shift in the SPR angle. Human interferon (IFN)-γ at concentrations ranging from 0.01 to 100 ng/ml was detectable by this method. We also show that this biocatalytic signal amplification method can be applied to SPR imaging (SPRI), in an immunoassay of multiple proteins on a protein microarray format.
- ISSN
- 0022-1759
- Publisher
- Elsevier
- Full Text Link
- http://dx.doi.org/10.1016/j.jim.2004.12.007
- Type
- Article
- Appears in Collections:
- Division of Research on National Challenges > Bionanotechnology Research Center > 1. Journal Articles
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