Enhanced sensitivity of surface plasmon resonance (SPR) immunoassays using a peroxidase-catalyzed precipitation reaction and its application to a protein microarray

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Title
Enhanced sensitivity of surface plasmon resonance (SPR) immunoassays using a peroxidase-catalyzed precipitation reaction and its application to a protein microarray
Author(s)
Min-Gon Kim; Yong Beom Shin; Jin Mi Jung; H S Ro; Bong Hyun Chung
Bibliographic Citation
Journal of Immunological Methods, vol. 297, no. 1, pp. 125-132
Publication Year
2005
Abstract
We describe a method to improve the sensitivity of surface plasmon resonance (SPR) immunoassays using a horseradish peroxidase (HRP)-catalyzed precipitation reaction. The precipitation reaction catalyzed by HRP bound to the SPR biosensor surface via a sandwich immunoassay induced a shift in the SPR angle. Human interferon (IFN)-γ at concentrations ranging from 0.01 to 100 ng/ml was detectable by this method. We also show that this biocatalytic signal amplification method can be applied to SPR imaging (SPRI), in an immunoassay of multiple proteins on a protein microarray format.
Keyword
Horseradish peroxidaseImmunoassayInterferon-γInterleukin-5Surface plasmon resonance (SPR)
ISSN
0022-1759
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.jim.2004.12.007
Type
Article
Appears in Collections:
Division of Biomaterials Research > Bionanotechnology Research Center > 1. Journal Articles
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