A novel function of benzyl isothiocyanate in vascular smooth muscle cells: The role of ERK1/2, cell cycle regulation, and matrix metalloproteinase-9

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Title
A novel function of benzyl isothiocyanate in vascular smooth muscle cells: The role of ERK1/2, cell cycle regulation, and matrix metalloproteinase-9
Author(s)
J Y Lee; S K Moon; C W Hwang; K S Nam; Y K Kim; H D Yoon; Min-Gon Kim; C H Kim
Bibliographic Citation
Journal of Cellular Physiology, vol. 203, no. 3, pp. 493-500
Publication Year
2005
Abstract
Dietary isothiocyanates (ITCs) have shown protective effects against certain chemically induced cancers in animal models. These inhibitory effects are associated with reduced levels of extracellular signal-regulated kinase (ERK) 1/2 activity and the arrest of the G1 cell cycle. Benzyl isothiocyanate (BITC) treatment down-regulates cyclins and CDKs and up-regulates the expression of the CDK inhibitor p21, but up-regulation of p27 or p53 was not detected. Since antiatherogenic effects are not needed for antiproliferation, we determined whether BITC exerted inhibitory effects on matrix metalloproteinase-9(MMP-9) activity in TNF-α-induced vascular smooth muscle cells (VSMCs). BITC inhibited TNF-α-induced MMP-9 secretion in VSMC in a dose dependent manner. This inhibition was characterized by the down-regulation of MMP-9, which is transcriptionally regulated at the NF-κB site, and the activation protein-1 (AP-1) site in the MMP-9 promoter. These findings indicate that BITC is an effective agent for inhibiting cell proliferation, the G1 to S phase cell cycle progress, and MMP-9 expression through the transcription factors NF-κB and AP-1 in TNF-α-induced VSMC.
ISSN
0021-9541
Publisher
Wiley
DOI
http://dx.doi.org/10.1002/jcp.20257
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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