C-terminal serine rich region of IKK-β interacts with Bfl-1, a homologue of Bcl-2 = Bcl-2 유사인자 Bfl-1과 결합하는 IKK-β의 C말단 Ser부위
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- C-terminal serine rich region of IKK-β interacts with Bfl-1, a homologue of Bcl-2 = Bcl-2 유사인자 Bfl-1과 결합하는 IKK-β의 C말단 Ser부위
- Hyun Kyung Yoon; Jin Koo Kim; Hee Jun Cho; Mi Young Cho; Yuk-Pheel Park; K H You; J W Kim; Do Young Yoon; Yong Kyung Choe; Hee Gu Lee
- Bibliographic Citation
- Korean Journal of Laboratory Medicine, vol. 25, no. 3, pp. 192-198
- Publication Year
- Background : Bcl-2 family proteins play a central role in regulating apoptosis. In human, over 20 members of this family have been identified to date. Bfl-1, a member of the Bcl-2 family, has been known to retard apoptosis in various cell lines. However, the function of Bfl-1 remains unclear. Methods : In order to investigate the Bfl-1 function, we employed yeast two-hybrid system to identify the proteins which are capable of interacting with Bfl-1. The interaction of inhibitor kappaB kinase- (IKK- ) and Bfl-1 was confirmed using glutathione S-transferase pull down assays. To determine which regions of IKK- were required for interaction with Bfl-1, we constructed 12 deletion mutants of IKK- and 5 deletion mutants of Bfl-1. Results : Bfl-1 interacted with the C-terminal region of IKK- which is a subunit of IKK complex, and IKK- activity is very important in the NF- B related pathway. In addition, the amino acids 673-745 of IKK- were important for Bfl-1 interactions, and amino acids 1-484 of Bfl-1, including Bcl-2 homology domains (BH1, BH2, BH3, BH4), were crucial for IKK- interactions. Conclusions : IKK C-terminus contains many serine residues as binding partner of Bfl-1. Our results suggested that Bfl-1 is involved in the NF- B activation through interaction of IKK- and Bfl-1. Further studies need to be performed to understand functions of the IKK- and Bfl-1 associated with the regulation of the NF- B activation pathway.
- Bfl-1IKK- yeast two hybridBH domain
- Korea Soc-Assoc-Inst
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- Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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