Isolation and characterization of a Rhodococcus species strain able to grow on ortho- and para-Xylene

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Title
Isolation and characterization of a Rhodococcus species strain able to grow on ortho- and para-Xylene
Author(s)
J Y Jang; Dockyu Kim; H W Bae; K Y Choi; J C Chae; G J Zylstra; Y M Kim; E Kim
Bibliographic Citation
Journal of Microbiology, vol. 43, no. 4, pp. 325-330
Publication Year
2005
Abstract
Rhodococcus sp. strain YU6 was isolated from soil for the ability to grow on o-xylene as the sole carbon and energy source. Unlike most other o-xylene-degrading bacteria, YU6 is able to grow on p-xylene. Numerous growth substrate range experiments, in addition to the ring-cleavage enzyme assay data, suggest that YU6 initially metabolizes o- and p-xylene by direct aromatic ring oxidation. This leads to the formation of dimethylcatechols, which was further degraded largely through meta-cleavage pathway. The gene encoding meta-cleavage dioxygenase enzyme was PCR cloned from genomic YU6 DNA using previously known gene sequence data from the o-xylene-degrading Rhodococcus sp. strain DK17. Subsequent sequencing of the 918-bp PCR product revealed a 98% identity to the gene, encoding methylcatechol 2,3-dioxygenase from DK17. PFGE analysis followed by Southern hybridization with the catechol 2,3-dioxygenase gene demonstrated that the gene is located on an approximately 560-kb megaplasmid, designated pJYJ1.
Keyword
catechol 2,3-dioxygenasemegaplasmidrhodococcusxylene isomers
ISSN
1225-8873
Publisher
Microbiological Society Korea
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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