Silencing of NbNAP1 encoding a plastidic SufB-like protein affects chloroplast development in Nicotiana benthamiana

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Title
Silencing of NbNAP1 encoding a plastidic SufB-like protein affects chloroplast development in Nicotiana benthamiana
Author(s)
C S Ahn; Jeong Hee Lee; H S Pai
Bibliographic Citation
Molecules and Cells, vol. 20, no. 1, pp. 112-118
Publication Year
2005
Abstract
It was previously shown that AtNAP1 is a plastidic SufB protein involved in Fe-S cluster assembly in Arabidopsis. In this study, we investigated the effects of depleting SufB protein from plant cells using virus-induced gene silencing (VIGS). VIGS of NbNAP1 encoding a Nicotiana benthamiana homolog of AtNAP1 resulted in a leaf yellowing phenotype. NbNAP1 was expressed ubiquitously in plant tissues with the highest level in roots. A GFP fusion protein of the N-terminal region (M1-V103) of NbNAP1 was targeted to chloroplasts. Depletion of NbNAP1 resulted in reduced numbers of chloroplasts of reduced size. Mitochondria also seemed to be affected. Despite the reduced number and size of the chloroplasts in the NbNAP1 VIGS lines, the expression of many nuclear genes encoding chloroplast-targeted proteins and chlorophyll biosynthesis genes remained unchanged.
Keyword
ABC Proteinchloroplast localizationFe-S clusterNbNAP1plastid factorvirus-induced gene silencing
ISSN
1016-8478
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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