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- Title
- Binding and regulation of hypoxia-inducible factor-1 by the inhibitory PAS proteins = 저해성 PAS 단백질에 의한 HIF-1a 단백질의 조절
- Author(s)
- Mi Sun Jang; Jae Eun Park; Jung A Lee; Sung Goo Park; P K Myung; Do Hee Lee; Byoung Chul Park; Sayeon Cho
- Bibliographic Citation
- Biochemical and Biophysical Research Communications, vol. 337, no. 1, pp. 209-215
- Publication Year
- 2005
- Abstract
- Hypoxia-inducible factor-1 (HIF-1), which consists of oxygen-sensitive HIF-1α and constitutively expressed HIF-1β subunits, activates transcription of genes encoding proteins that mediate adaptive responses to reduced oxygen availability. The mouse inhibitory PAS (Per/Arnt/Sim) domain protein (IPAS) functions as a negative regulator in HIF-mediated gene expression. In this report, we cloned the human orthologs of the mouse IPAS gene, IPASH1 and IPASH2, to further study the regulatory mechanism of HIF-1 by the IPAS proteins. The human IPAS proteins inhibited the transactivation function of HIF-1α under hypoxic conditions. In addition, human IPAS proteins blocked the hypoxia-induced VEGF expression and inhibited cell migration and tube formation of human umbilical vein endothelial cells. Interestingly, both HIF-1α and HIF-1β interacted with the IPAS proteins. Collectively, these results suggest that human IPAS proteins inhibit angiogenesis by binding to and inhibiting HIF-1α and HIF-1β.
- Keyword
- AngiogenesisHypoxiaHypoxia-inducible factor-1αInhibitory PAS domain protein
- ISSN
- 0006-291X
- Publisher
- Elsevier
- DOI
- http://dx.doi.org/10.1016/j.bbrc.2005.09.038
- Type
- Article
- Appears in Collections:
- Division of Biomedical Research > Disease Target Structure Research Center > 1. Journal Articles
Critical Diseases Diagnostics Convergence Research Center > 1. Journal Articles
- Files in This Item:
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