Investigation of possible horizontal gene transfer from the leaf tissue of transgenic potato to soil vacteria

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Title
Investigation of possible horizontal gene transfer from the leaf tissue of transgenic potato to soil vacteria
Author(s)
Young Tae Kim; Sung Eun Kim; Ki Duk Park; Tae Hoon Kang; Yun Mi Lee; S H Lee; Jae Sun Moon; Sung Uk Kim
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 15, no. 5, pp. 1130-1134
Publication Year
2005
Abstract
To monitor the possibility of horizontal gene transfer between transgenic potato and bacteria in the environment, the gene flow from glufosinate-tolerant potato to bacteria in soils was investigated. The soil samples treated with the leaf tissue of either glufosinate-tolerant or glufosinate-sensitive potato were subjected to PCR and Southern hybridization to determine possible occurrence of glufosinate-resistant soil bacteria and to detect the bar (phosphinothricin acetyltransferase) gene, conferring tolerance to glufosinate. The bar gene was not detected from genomic DNAs extracted at different time intervals from the soil samples, which had been treated with the leaf tissue of either transgenic or nontransgenic potato for 2 to 8 weeks. In addition, the level of glufosinate-resistant bacteria isolated from the soil samples treated with the leaf tissue of transgenic potato was similar to that of the samples treated with non-transgenic potato after 4 months of incubation at 25°C. The bar gene was not detected in the genomic DNAs extracted from colonies growing on the plate containing glufosinate, indicating that the bacteria could acquire the resistant phenotype to glufosinate by another mechanism without the uptake of the bar gene from glufosinate-tolerant potato.
Keyword
environmental risk assessmentglufosinateherbicide tolerancehorizontal gene transfermicrocosmtransgenic potato
ISSN
1017-7825
Publisher
South Korea
Type
Article
Appears in Collections:
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
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