Development and evaluation of genome-probing microarrays for monitoring lactic acid bacteria = 김치 내 유산균 발효과정 모니터링을 위한 Genome-Probing Microarrays(GPM)의 개발

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Title
Development and evaluation of genome-probing microarrays for monitoring lactic acid bacteria = 김치 내 유산균 발효과정 모니터링을 위한 Genome-Probing Microarrays(GPM)의 개발
Author(s)
Jin-Woo Bae; S K Rhee; Ja Ryeong Park; Won Hyong Chung; Young Do Nam; I Lee; H Kim; Yong Ha Park
Bibliographic Citation
Applied and Environmental Microbiology, vol. 71, no. 12, pp. 8825-8835
Publication Year
2005
Abstract
The genome-probing microarray (GPM) was developed for quantitative, high-throughput monitoring of community dynamics in lactic acid bacteria (LAB) fermentation through the deposit of 149 microbial genomes as probes on a glass slide. Compared to oligonucleotide microarrays, the specificity of GPM was remarkably increased to a species-specific level. GPM possesses about 10- to 100-fold higher sensitivity (2.5 ng of genomic DNA) than the currently used 50-mer oligonucleotide microarrays. Since signal variation between the different genomes was very low compared to that of cDNA or oligonucleotide-based microarrays, the capacity of global quantification of microbial genomes could also be observed in GPM hybridization. In order to assess the applicability of GPMs, LAB community dynamics were monitored during the fermentation of kimchi, a traditional Korean food. In this work, approximately 100 diverse LAB species could be quantitatively analyzed as actively involved in kimchi fermentation.
ISSN
0099-2240
Publisher
Amer Soc Microb
DOI
http://dx.doi.org/10.1128/AEM.71.12.8825-8835.2005
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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