TEM sample preparation for cultured neurons on a glass coverslip = Hydrofluoric acid 용액을 이용한 유리 커버글라스에 배양된 신경세포의 전자현미경 시료제작법

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Title
TEM sample preparation for cultured neurons on a glass coverslip = Hydrofluoric acid 용액을 이용한 유리 커버글라스에 배양된 신경세포의 전자현미경 시료제작법
Author(s)
Hyun Woo OhHo Yong Park
Bibliographic Citation
Korean Journal of Electron Microscopy, vol. 35, no. 4, pp. 197-202
Publication Year
2005
Abstract
Cultured neurons from Drosophila brain on a glass coverslip to understand the structural basis of synapse were prepared for TEM observations. Neurons on a coverslip were fixed, dehydrated and embedded in Epon without separating from coverslip. After polymerization, the block was placed in 49% hydrofluoric acid to remove the coverslip. The block was examined under a light microscope to select exact neurons, then trimmed and sectioned for TEM observation.
Keyword
cell cultureglass coversliphydrofluoric acidsynapse
ISSN
I000-0077
Publisher
South Korea
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > Core Facility Management Center > 1. Journal Articles
Division of Biomaterials Research > Industrial Bio-materials Research Center > 1. Journal Articles
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