Identification of target proteins of N-acetylglucosaminyl transferase V in human colon cancer and implications of protein tyrosine phosphatase kappa in enhanced cancer cell migration
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- Identification of target proteins of N-acetylglucosaminyl transferase V in human colon cancer and implications of protein tyrosine phosphatase kappa in enhanced cancer cell migration
- Yong Sam Kim; Hye Yeon Kang; J Y Kim; S Oh; C H Kim; Chun Jeih Ryu; E Miyoshi; N Taniguchi; Jeong Heon Ko
- Bibliographic Citation
- Proteomics, vol. 6, no. 4, pp. 1187-1191
- Publication Year
- To gain a better understanding of the mechanism underlying colon cancer and to search for potential markers of colon cancer prognosis, a comparative proteomic analysis of colon cancer WiDr cells was conducted using 2-DE and lectin blot, followed by identification based on ESI-MS. Through these approaches 14 proteins were identified as candidate target proteins for N-acetylglucosaminyl transferase V (GnT-V) that would be expected to be implicated in the progression of colon cancer. We selected protein tyrosine phosphatase kappa (PTPκ) as a model protein to validate this approach to the discovery of novel biomarkers in colon cancer. PTPκ underwent an aberrant glycosylation in GnT-V-overexpressing WiDr cells, and the aberrantly glycosylated PTPκ was vulnerable to proteolytic cleavage. The enhanced cleavage of PTPκ in GnT-V-overexpressing cells was responsible for the mitigation of the homophilic binding capacity, resulting in an increase in cancer cell migration.
- 2-DE; colon cancer; glycomics; N-Acetylglucosaminyl transferase V; protein tyrosine phosphatase κ
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- Division of Biomedical Research > Genome Editing Research Center > 1. Journal Articles
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