Surface plasmon resonance imaging protein arrays for analysis of triple protein interactions of HPV, E6, E6AP, and p53 = HPV, E6, E6AP, p53 상호작용 연구를 위한 SPR 이미징 단백질 어레이

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dc.contributor.authorH-S Ro-
dc.contributor.authorByung Hoon Koh-
dc.contributor.authorSun Ok Jung-
dc.contributor.authorHyun Kyu Park-
dc.contributor.authorYong Beom Shin-
dc.contributor.authorMin-Gon Kim-
dc.contributor.authorBong Hyun Chung-
dc.date.accessioned2017-04-19T09:04:14Z-
dc.date.available2017-04-19T09:04:14Z-
dc.date.issued2006-
dc.identifier.issn1615-9853-
dc.identifier.uri10.1002/pmic.200500635ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/7343-
dc.description.abstractWe have developed a surface plasmon resonance (SPR)-based protein microarray to study protein-protein interactions in a high-throughput mode. As a model system, triple protein interactions have been explored with human papillomaviral E6 protein, tumor suppressor p53, and ubiquitin ligase E6AP. Human papillomavirus (HPV) is known to be a causative agent of cervical cancer. Upon infection, the viral E6 protein forms a heterotrimeric protein complex with p53 and E6AP. The formation of the complex eventually results in the degradation of p53. In the present study, a GST-fused E6AP protein was layered onto a glutathione (GSH)-modified gold chip surface. The specific binding of GST-E6AP protein onto the gold chip surface was facilitated through the affinity of GST to its specific ligand GSH. The interacting proteins (E6 and/or p53) were then spotted. Detection of the interaction was performed using a SPR imaging (SPRI) technique. The resulting SPRI intensity data showed that the protein-protein interactions of E6AP, E6, and p53 were detected in a concentration-dependent manner, suggesting that the SPRI-based microarray system can be an effective tool to study protein-protein interactions where multiple proteins are involved.-
dc.publisherWiley-
dc.titleSurface plasmon resonance imaging protein arrays for analysis of triple protein interactions of HPV, E6, E6AP, and p53 = HPV, E6, E6AP, p53 상호작용 연구를 위한 SPR 이미징 단백질 어레이-
dc.title.alternativeSurface plasmon resonance imaging protein arrays for analysis of triple protein interactions of HPV, E6, E6AP, and p53-
dc.typeArticle-
dc.citation.titleProteomics-
dc.citation.number7-
dc.citation.endPage2111-
dc.citation.startPage2108-
dc.citation.volume6-
dc.contributor.affiliatedAuthorByung Hoon Koh-
dc.contributor.affiliatedAuthorSun Ok Jung-
dc.contributor.affiliatedAuthorHyun Kyu Park-
dc.contributor.affiliatedAuthorYong Beom Shin-
dc.contributor.affiliatedAuthorMin-Gon Kim-
dc.contributor.affiliatedAuthorBong Hyun Chung-
dc.contributor.alternativeName노현수-
dc.contributor.alternativeName고병훈-
dc.contributor.alternativeName정선옥-
dc.contributor.alternativeName박현규-
dc.contributor.alternativeName신용범-
dc.contributor.alternativeName김민곤-
dc.contributor.alternativeName정봉현-
dc.identifier.bibliographicCitationProteomics, vol. 6, no. 7, pp. 2108-2111-
dc.identifier.doi10.1002/pmic.200500635-
dc.subject.keywordhuman papillomavirus (HPV)-
dc.subject.keywordprotein arrays-
dc.subject.keywordprotein interaction-
dc.subject.keywordsurface plasmon resonance imaging-
dc.subject.localhuman papillomavirus (HPV)-
dc.subject.localHuman papillomavirus-
dc.subject.localhuman paillomavirus(HPV)-
dc.subject.localhuman papillomavirus-
dc.subject.localHuman papillomavirus (HPV)-
dc.subject.localhuman papilloma virus (HPV)-
dc.subject.localHPV-
dc.subject.localProtein arrays-
dc.subject.localprotein arrays-
dc.subject.localprotein interaction-
dc.subject.localProtein interaction-
dc.subject.localSurface plasmon resonance imaging (SPRI)-
dc.subject.localsurface plasmon resonance imaging-
dc.subject.localSurface plasmon resonance (SPR) imaging-
dc.subject.localsurface plasmon resonance imaging (SPRI)-
dc.subject.localSurface plasmon resonance imaging-
dc.subject.localsurface plasmon resonance (SPR) imaging-
dc.description.journalClassY-
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Division of Research on National Challenges > Bionanotechnology Research Center > 1. Journal Articles
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