Surface plasmon resonance imaging protein arrays for analysis of triple protein interactions of HPV, E6, E6AP, and p53 = HPV, E6, E6AP, p53 상호작용 연구를 위한 SPR 이미징 단백질 어레이
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- Title
- Surface plasmon resonance imaging protein arrays for analysis of triple protein interactions of HPV, E6, E6AP, and p53 = HPV, E6, E6AP, p53 상호작용 연구를 위한 SPR 이미징 단백질 어레이
- Author(s)
- H-S Ro; Byung Hoon Koh; Sun Ok Jung; Hyun Kyu Park; Yong Beom Shin; Min-Gon Kim; Bong Hyun Chung
- Bibliographic Citation
- Proteomics, vol. 6, no. 7, pp. 2108-2111
- Publication Year
- 2006
- Abstract
- We have developed a surface plasmon resonance (SPR)-based protein microarray to study protein-protein interactions in a high-throughput mode. As a model system, triple protein interactions have been explored with human papillomaviral E6 protein, tumor suppressor p53, and ubiquitin ligase E6AP. Human papillomavirus (HPV) is known to be a causative agent of cervical cancer. Upon infection, the viral E6 protein forms a heterotrimeric protein complex with p53 and E6AP. The formation of the complex eventually results in the degradation of p53. In the present study, a GST-fused E6AP protein was layered onto a glutathione (GSH)-modified gold chip surface. The specific binding of GST-E6AP protein onto the gold chip surface was facilitated through the affinity of GST to its specific ligand GSH. The interacting proteins (E6 and/or p53) were then spotted. Detection of the interaction was performed using a SPR imaging (SPRI) technique. The resulting SPRI intensity data showed that the protein-protein interactions of E6AP, E6, and p53 were detected in a concentration-dependent manner, suggesting that the SPRI-based microarray system can be an effective tool to study protein-protein interactions where multiple proteins are involved.
- Keyword
- human papillomavirus (HPV)protein arraysprotein interactionsurface plasmon resonance imaging
- ISSN
- 1615-9853
- Publisher
- Wiley
- DOI
- http://dx.doi.org/10.1002/pmic.200500635
- Type
- Article
- Appears in Collections:
- Division of Research on National Challenges > Bionanotechnology Research Center > 1. Journal Articles
- Files in This Item:
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