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- Inhibition of diacylglycerol acyltransferase by betulinic acid from Alnus hirsuta
- Mi Yeon Chung; Mun Chual Rho; Seung Woong Lee; Hye Ran Park; K Kim; I A Lee; D H Kim; K H Jeune; Hyun Sun Lee; Young Kook Kim
- Bibliographic Citation
- Planta Medica, vol. 72, no. 3, pp. 267-269
- Publication Year
- During the screening for diacylglycerol acyltransferase (DGAT) inhibitors from natural products, the lupane-type triterpenoid betulinic acid was isolated from the methanol extract of Alnus hirsuta. It potently inhibited DGAT in the rat liver microsomes with an IC50 value of 9.6 μM. Enzyme kinetic studies showed apparent Km and Ki values of 13.3 μM and 8.1 μM using [14C]oleoyl-CoA as a substrate. A decrease in the apparent Vmax was observed with betulinic acid, whereas the apparent Km remained constant. Therefore, a Lineweaver-Burk plot of DGAT inhibition by betulinic acid showed a non-competitive type of inhibition. In the cell-based assay, betulinic acid inhibited triglyceride (TG) formation by human HepG2 cells. These findings suggest that betulinic acid may be a potential lead compound in the treatment of obesity.
- Georg Thieme Verlag Kg
- Appears in Collections:
- Jeonbuk Branch Institute > Immunoregulatory materials Research Center > 1. Journal Articles
Ochang Branch Institute > Natural Medicine Research Center > 1. Journal Articles
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