Enhanced reusability of hexa-arginine-tagged esterase immobilized on gold-coated magnetic nanoparticles

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Title
Enhanced reusability of hexa-arginine-tagged esterase immobilized on gold-coated magnetic nanoparticles
Author(s)
Jin Young JeongTai-Hwan Ha; Bong Hyun Chung
Bibliographic Citation
Analytica Chimica Acta, vol. 569, pp. 203-209
Publication Year
2006
Abstract
Gold-coated magnetic nanoparticle (GMP) was manufactured and used as a carrier for the immobilization of hexa-arginine-tagged esterase (Arg6-esterase). The formation of gold shell on the magnetic nanoparticle (Fe2O3) was performed by an iterative reduction method using hydroxylamine as a reductant. The surface of the GMP was further functionalized with mercapto-hexadecanoic acid (MHA) to tether the positively charged Arg6-esterase effectively. The enzymatic activity of the immobilized Arg6-esterase was investigated by monitoring the dissociation rate of its colorimetric substrate, p-nitrophenol butyrate (pNPB). Although the immobilized enzyme exhibited a lower activity (∼60%) compared to the free one, its original activity was found to be retained even after seven times repetitive uses by magnetic decantation.
Keyword
Enzyme immobilizationGold-coated magnetic nanoparticle (GMP)Hexa-arginine-tagged esterase (Arg6-esterase)Magnetic separation
ISSN
0003-2670
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.aca.2006.03.102
Type
Article
Appears in Collections:
Division of Research on National Challenges > Environmental diseases research center > 1. Journal Articles
Division of Bio Technology Innovation > Core Research Facility & Analysis Center > 1. Journal Articles
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