Preparation of soluble silk peptides by food-grade proteinases = 효소분해에 의한 가용성 실크 펩타이드의 제조

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Title
Preparation of soluble silk peptides by food-grade proteinases = 효소분해에 의한 가용성 실크 펩타이드의 제조
Author(s)
Jae Seok Ha; Jae Jun Song; H K Cho; Seung Goo Lee
Bibliographic Citation
Korean Journal of (Applied) Microbiology & Biotechnology, vol. 34, no. 2, pp. 115-120
Publication Year
2006
Abstract
Enzymatic hydrolysis of silk fibers were investigated for the preparation of soluble silk peptides by ten food-grade proteases from Bacillus, Aspergillus, and plant sources. Silk fibers were dissolved for 1 hr in a 2:1 cosolvent (50% CaCl2: ethanol) by heating at 90°C. The silk solution was filtered to remove impurity particles and desalted for 50 hours by a dialysis process to remove the used cosolvent. When the silk hydrolysis was performed at 45°C for 2 hours, most proteases from Bacillus and Aspergillus generated large amounts of insoluble aggregates. On the contrary, proteases from plant sources produced much less aggregates during prolonged incubations and also exhibited high hydrolysis activities. In regards of the solubility and broad molecular sizes of produced silk peptides, Bromelain was finally selected and applied for the enzymatic hydrolysis of silk fibers.
Keyword
silk fibroinsoluble silk peptidesilkfood-grade protease
ISSN
0257-2389
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
Synthetic Biology and Bioengineering Research Institute > 1. Journal Articles
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