A potential demerit of the pronuclear microinjection technique

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Title
A potential demerit of the pronuclear microinjection technique
Author(s)
A G Wang; Sun-Uk Kim; H B Moon; Byung Hwa Hyun; Ki Hoan Nam; J G Suh; Nam-Soon Kim; Dae Yeul Yu; Dong Seok Lee
Bibliographic Citation
Korean Journal of Life Sciences, vol. 16, no. 4, pp. 566-570
Publication Year
2006
Abstract
Pronuclear microinjection (PMI) is a primary method for producing transgenic mice and offers a powerful tool for investigating gene function in vivo. The method has several reported advantages and disadvantages. Here, we report another potential shortcoming. The survival rate of fertilized one cell-stage embryos was significantly reduced after PMI procedure (65.4% (1202/1838)). In addition, the proportion of embryos developing to full-term was also significantly lower than that of embryos not undergoing PMI (26.5% (319/1202) vs 41.9% (57/136)). Moreover, 3 out of 21 (14.3%) founder control mice which were non-transgene-carrying littermates of transgenic founders showed histopathological changes in their liver, which was comparable to that in of transgenic lineages (4 out of 27 (14.8%)). In conclusion, the mechanical damages in chromosomes occurring during PMI procedure may be a potential factor influencing phenotypes of transgenic mice.
Keyword
nuclear damagepronuclear microinjectiontransgenic mice
ISSN
I000-0052
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.5352/JLS.2006.16.4.566
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of National Bio-Infrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
Ochang Branch Institute > Division of National Bio-Infrastructure > Laboratory Animal Resource & Research Center > 1. Journal Articles
Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
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