Development of bioreactor system for L-tyrosine synthesis using thermostable tyrosine phenol-lyase = 내열성 tyrosine phenol-lyase를 이용한 L-tyrosine 합성 생물반응시스템의 개발

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Title
Development of bioreactor system for L-tyrosine synthesis using thermostable tyrosine phenol-lyase = 내열성 tyrosine phenol-lyase를 이용한 L-tyrosine 합성 생물반응시스템의 개발
Author(s)
Do Young KimEugene Rha; Su-Lim Choi; Jae Jun Song; S P Hong; M H Sung; Seung Goo Lee
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 17, no. 1, pp. 116-122
Publication Year
2007
Abstract
An efficient enzyme system for the synthesis of L-tyrosine was developed using a fed-batch reactor with continuous feeding of phenol, pyruvate, and ammonia. A thermo- and chemostable tyrosine phenol-lyase from Symbiobacterium toebii was employed as the biocatalyst in this work. The enzyme was produced using a constitutive expression system in Escherichia coli BL21, and prepared as a soluble extract by rapid clarification, involving treatment with 40% methanol in the presence of excess ammonium chloride. The stability of the enzyme was maintained for at least 18 h under the synthesis conditions, including 75 mM phenol at pH 8.5 and 40°C. The fed-batch system (working volume, 0.5 ?1) containing 1.0 kU of the enzyme preparation was continuously fed with two substrate preparations: one containing 2.2 M phenol and 2.4 M sodium pyruvate, and the other containing 0.4 mM pyridoxal-5-phosphate and 4 M ammonium chloride (pH 8.5). The system produced 130 g/l of L-tyrosine within 30 h, mostly as precipitated particles, upon continuous feeding of the substrates for 22 h. The maximum conversion yield of L-tyrosine was 94% on the basis of the supplied phenol.
Keyword
bioconversionfed-batchL-tyrosinesymbiobacterium toebiityrosine phenol-lyase
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Division of Biomedical Research > Microbiome Convergence Research Center > 1. Journal Articles
Synthetic Biology and Bioengineering Research Institute > Synthetic Biology Research Center > 1. Journal Articles
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
Synthetic Biology and Bioengineering Research Institute > 1. Journal Articles
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