Monoclonal anti-thrombopoietin antibodies generated by genetic immunization

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Title
Monoclonal anti-thrombopoietin antibodies generated by genetic immunization
Author(s)
N K Lim; J H Kim; S Y Kim; H J Kang; K S Kim; S Lee; Hyo Jeong Hong; K S Inn
Bibliographic Citation
Hybridoma, vol. 25, no. 2, pp. 75-79
Publication Year
2006
Abstract
Thrombopoietin (TPO) is a megakaryocyte growth and differentiation factor that is currently being investigated as a therapeutic for cancer patients undergoing myelosuppressive chemotherapy. We generated monoclonal antibodies (MAbs) specific for human thrombopoietin (hTPO) by genetic immunization using an hTPO expression plasmid and an adjuvant plasmid that encodes mouse granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4). All genetically immunized mice exhibited a high humoral immune response. Splenocytes from these mice were used to generate hybridomas. Two MAbs, designated 2B9A10 and 4C16B15 (of IgG1 and IgG3 isotypes, respectively), were subsequently selected and produced. They specifically recognized and precipitated recombinant hTPO produced by mammalian cells and were effective in sandwich enzyme-linked immunosorbent assays (ELISAs) for hTPO quantitation. Our results demonstrate that these MAbs should be useful for purification and quantitation of hTPO in clinical and laboratory settings.
ISSN
0272-457X
Publisher
Mary Ann Liebert
DOI
http://dx.doi.org/10.1089/hyb.2006.25.75
Type
Article
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1. Journal Articles > Journal Articles
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