Dynamic changes of gangliosides expression during the differentiation of embryonic and mesenchymal stem cells into neural cells

Cited 36 time in scopus
Metadata Downloads
Title
Dynamic changes of gangliosides expression during the differentiation of embryonic and mesenchymal stem cells into neural cells
Author(s)
D H Kwak; Kweon Yu; S M Kim; D H Lee; S M Kim; J U Jung; J W Seo; N Kim; S Lee; K Y Jung; H K You; H A Kim; Y K Choo
Bibliographic Citation
Experimental and Molecular Medicine, vol. 38, no. 6, pp. 668-676
Publication Year
2006
Abstract
Stem cells are used for the investigation of developmental processes at both cellular and organism levels and offer tremendous potentials for clinical applications as an unlimited source for transplantation. Gangliosides, sialic acid-conjugated glycosphingolipids, play important regulatory roles in cell proliferation and differentiation. However, their expression patterns in stem cells and during neuronal differentiation are not known. Here, we investigated expression of gangliosides during the growth of mouse embryonic stem cells (mESCs), mesenchymal stem cells (MSCs) and differentiated neuronal cells by using high-performance thin-layer chromatography (HPTLC). Monosialoganglioside 1 (GM1) was expressed in mESCs and MSCs, while GM3 and GD3 were expressed in embryonic bodies. In the 9-day old differentiated neuronal cells from mESCs cells and MSCs, GM1 and GT1b were expressed. Results from immunostaining were consistent with those observed by HPTLC assay. These suggest that gangliosides are specifically expressed according to differentiation of mESCs and MSCs into neuronal cells and expressional difference of gangliosides may be a useful marker to identify differentiation of mESCs and MSCs into neuronal cells.
Keyword
Cell differentiationEmbryonic developmentFluorescent antibody techniquesGangliosidesMesenchymal stem cellsStem cells
ISSN
I000-0028
Publisher
Springer-Nature Pub Group
DOI
http://dx.doi.org/10.1038/emm.2006.79
Type
Article
Appears in Collections:
Division of Biomedical Research > Disease Target Structure Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.