Expression of thermostable α-glucosidase from Thermus caldophilus GK24 in recombinant Saccharomyces cerevisiae

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Title
Expression of thermostable α-glucosidase from Thermus caldophilus GK24 in recombinant Saccharomyces cerevisiae
Author(s)
J Y Choi; Jungoh Ahn; S I Kim; H J Shin
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 16, no. 12, pp. 2000-2003
Publication Year
2006
Abstract
A gene (GenBank AF096282) coding for a α-glucosidase (TcaAG, EC 3.2.1.20) from Thermus caldophilus GK24 was expressed in Saccharomyces cerevisiae, a generally recognized as safe (GRAS) host. The thermostable α-glucosidase was produced inside of the GRAS host at 0.04 unit/ mg-dry cell by the constitutively expressing ADH1 promoter and at 1.2 unit/ mg-dry cell by the inductively expressing GAL10 promoter, respectively. No α-glucosidase activities were found in the medium when the MF-alpha signal sequence from S. cerevisiae or α-amylase signal sequence from Aspergillus oryzae were fused before the α-glucosidase gene for the secretion.
Keyword
α-Glucosidaseprotein expressionrecombinant enzymesaccharomyces cerevisiaethermus caldosphilus Gk24
ISSN
1017-7825
Publisher
South Korea
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
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