Horseradish peroxidase (HRP) immobilized poly(aniline-co-m-aminophenol) film electrodes-fabrication and evaluation as hydrogen peroxide sensor

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dc.contributor.authorK D Seo-
dc.contributor.authorK P Lee-
dc.contributor.authorA I Gopalan-
dc.contributor.authorSang Jeon Chung-
dc.contributor.authorYong Taik Lim-
dc.contributor.authorS H Choi-
dc.date.accessioned2017-04-19T09:07:36Z-
dc.date.available2017-04-19T09:07:36Z-
dc.date.issued2007-
dc.identifier.issn1424-8220-
dc.identifier.uri10.3390/s7050719ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/7957-
dc.description.abstractEnzyme modified electrodes were fabricated with poly(aniline-co-m- aminophenol). Electrochemical polymerization of aniline and m-aminophenol was performed to get the film of copolymer on the surface of gold electrode. Modified electrodes were fabricated by two methods, physical entrapment and covalent cross-linking. In one of the method, gold nanoparticles were loaded into the copolymer film and horseradish peroxidase (HRP) was immobilized into the Au nanoparticle loaded copolymer film through physical entrapment. In the other method, the amino and -OH groups in the copolymer are utilized to form covalent functionalization with HRP via glutaric dialdehyde as cross-linker/mediator. The conducting copolymer/enzyme modified electrodes prepared by physical entrapment/covalent functionalization of enzyme were tested for electrocatalytic activities towards sensing of H2O2. Amperometric results indicate that enzyme modified electrode via physical entrapment possesses better electrocatalytic performance over covalent functionalized enzyme electrode.-
dc.publisherMDPI-
dc.titleHorseradish peroxidase (HRP) immobilized poly(aniline-co-m-aminophenol) film electrodes-fabrication and evaluation as hydrogen peroxide sensor-
dc.title.alternativeHorseradish peroxidase (HRP) immobilized poly(aniline-co-m-aminophenol) film electrodes-fabrication and evaluation as hydrogen peroxide sensor-
dc.typeArticle-
dc.citation.titleSensors-
dc.citation.number5-
dc.citation.endPage729-
dc.citation.startPage719-
dc.citation.volume7-
dc.contributor.affiliatedAuthorSang Jeon Chung-
dc.contributor.affiliatedAuthorYong Taik Lim-
dc.contributor.alternativeName서강덕-
dc.contributor.alternativeName이광필-
dc.contributor.alternativeNameGopalan-
dc.contributor.alternativeName정상전-
dc.contributor.alternativeName임용택-
dc.contributor.alternativeName최성호-
dc.identifier.bibliographicCitationSensors, vol. 7, no. 5, pp. 719-729-
dc.identifier.doi10.3390/s7050719-
dc.subject.keywordau nanoparticles-
dc.subject.keywordcovalent bonding-
dc.subject.keywordhorseradish peroxidase-
dc.subject.keywordphysical adsorption-
dc.subject.keywordpoly(aniline-co-m-aminophenol)-
dc.subject.localau nanoparticles-
dc.subject.localcovalent bonding-
dc.subject.localhorseradish peroxidase-
dc.subject.localHorseradish peroxidase-
dc.subject.localphysical adsorption-
dc.subject.localpoly(aniline-co-m-aminophenol)-
dc.description.journalClassY-
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