Horseradish peroxidase (HRP) immobilized poly(aniline-co-m-aminophenol) film electrodes-fabrication and evaluation as hydrogen peroxide sensor

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Title
Horseradish peroxidase (HRP) immobilized poly(aniline-co-m-aminophenol) film electrodes-fabrication and evaluation as hydrogen peroxide sensor
Author(s)
K D Seo; K P Lee; A I Gopalan; Sang Jeon Chung; Yong Taik Lim; S H Choi
Bibliographic Citation
Sensors, vol. 7, no. 5, pp. 719-729
Publication Year
2007
Abstract
Enzyme modified electrodes were fabricated with poly(aniline-co-m- aminophenol). Electrochemical polymerization of aniline and m-aminophenol was performed to get the film of copolymer on the surface of gold electrode. Modified electrodes were fabricated by two methods, physical entrapment and covalent cross-linking. In one of the method, gold nanoparticles were loaded into the copolymer film and horseradish peroxidase (HRP) was immobilized into the Au nanoparticle loaded copolymer film through physical entrapment. In the other method, the amino and -OH groups in the copolymer are utilized to form covalent functionalization with HRP via glutaric dialdehyde as cross-linker/mediator. The conducting copolymer/enzyme modified electrodes prepared by physical entrapment/covalent functionalization of enzyme were tested for electrocatalytic activities towards sensing of H2O2. Amperometric results indicate that enzyme modified electrode via physical entrapment possesses better electrocatalytic performance over covalent functionalized enzyme electrode.
Keyword
au nanoparticlescovalent bondinghorseradish peroxidasephysical adsorptionpoly(aniline-co-m-aminophenol)
ISSN
1424-8220
Publisher
MDPI
DOI
http://dx.doi.org/10.3390/s7050719
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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