Plant regeneration from zygotic embryo-derived embryogenic cell suspension cultures of Ranunculus kazusensis

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Title
Plant regeneration from zygotic embryo-derived embryogenic cell suspension cultures of Ranunculus kazusensis
Author(s)
Sung Ran Min; Jang Ryol Liu; Suk Weon Kim
Bibliographic Citation
Plant Biotechnology Reports, vol. 1, no. 1, pp. 57-60
Publication Year
2007
Abstract
Culture conditions for high frequency plant regeneration via somatic embryogenesis from cell suspension cultures of Ranunculus kazusensis are described. Zygotic embryos formed white nodular structures and pale-yellow calluses at a frequency of 84.9% when cultured on half-strength Schenk and Hildebrandt (SH) medium supplemented with 0.1 mg l.1 2,4-dichlorophenoxyacetic acid (2,4-D). However, the frequency of white nodular structure and off-white callus formation decreased with an increasing concentration of 2,4-D up to 10 mg l.1, when the frequency reached 25%. Cell suspension cultures were established from zygotic embryo-derived pale-yellow calluses using half-strength SH medium supplemented with 0.1 mg l.1 of 2,4-D. Upon plating onto halfstrength SH basal medium, over 90% of cell aggregates gave rise to numerous somatic embryos and developed into plantlets. Regenerated plantlets were successfully transplanted to potting soil and grown to maturity at a survival rate of over 90% in a growth chamber. The plant regeneration system established in this study can be applied to mass propagation and conservation of this species.
Keyword
ranunculus kazusensisplant regenerationsomatic embryogenesissuspension culture
ISSN
1863-5466
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s11816-006-0005-0
Type
Article
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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