Dynamic DNA methylation reprogramming: Active demethylation and immediate remethylation in the male pronucleus of bovine zygotes

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Title
Dynamic DNA methylation reprogramming: Active demethylation and immediate remethylation in the male pronucleus of bovine zygotes
Author(s)
Jung Sun Park; Young Sun Jeong; S T Shin; Kyung Kwang Lee; Yong-Kook Kang
Bibliographic Citation
Developmental Dynamics, vol. 236, no. 9, pp. 2523-2533
Publication Year
2007
Abstract
DNA methylation reprogramming (DMR) is believed to be a key process by which mammalian zygotes gain nuclear totipotency through erasing epigenetic modifications acquired during gametogenesis. Nonetheless, DMR patterns do not seem to be conserved among mammals. To identify uniform rules underlying mammalian DMRs, we explored DMRs of diverse mammalian zygotes. of the zygotes studied, of particular interest was the bovine zygote; the paternal DNA methylation first decreased and was then rapidly restored almost to the maternal methylation level even before the two-cell stage. The 5-azadeoxycytidine treatment led to complete demethylation of the male pronucleus. The unusually dramatic changes in DNA methylation levels indicate that the bovine male pronucleus undergoes active demethylation, which is followed by de novo methylation. Our results show that, in bovine, the compound processes of active DNA demethylation and de novo DNA methylation, along with de novo H3-K9 trimethylation also, take place altogether within this very narrow window of pronucleus development.
Keyword
Active demethylationBovine embryoDe novo methylationDNA methylationEpigeneticHistone methylationMale pronucleusReprogramming
ISSN
1058-8388
Publisher
Wiley
DOI
http://dx.doi.org/10.1002/dvdy.21278
Type
Article
Appears in Collections:
Division of Research on National Challenges > Aging Research Center > 1. Journal Articles
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