Development of a simple and rapid immunochromatographic strip test for diarrhea-causative porcine rotavirus in swine stool

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Title
Development of a simple and rapid immunochromatographic strip test for diarrhea-causative porcine rotavirus in swine stool
Author(s)
J H Kang; Dur Han Kwon; T W Chung; Y D Kim; Hee Gu LeeJae Wha Kim; In Seong Choe; K W Kim; J S Lim; Eun Young Song; C H Kim
Bibliographic Citation
Journal of Virological Methods, vol. 146, no. 1, pp. 74-79
Publication Year
2007
Abstract
A rapid and simple immunochromatography (IC) strip test, for specific detection of porcine rotavirus (PRV) in stool specimen, was developed. Monoclonal antibodies (mAbs) to the OSU strain of PRV have been produced in mice. Among them, two hybridoma clones that generate mAb-1 and mAb-2, respectively, specific for VP6 protein of PRV, have been selected. In the IC configuration, mAb-1, one of the selected mAbs was used to the designed coat microparticles (MP), while another mAb-2 was used to fix it on the nitrocellulose membrane strip to form a result line. The control line was formed on the same membrane strip past the result line by fixing anti-mouse IgG antibody. The IC test was capable of detecting 1000 plaque-forming units of PRV/ml in less than 5 min, and the binding capacity was demonstrated by specific recognition of PRV only, but not other porcine diarrhea viruses, transmissible gastroenteritis virus (TGEV) and porcine epidemic diarrhea virus (PEDV). The IC test produced positive results with all the nine PRV-positive stool specimens and negative results with five different non-PRV specimens, which were identified previously by the polymerase chain reaction (PCR) test, respectively. The results indicate an excellent concordance between the two methods, suggesting a potential application of the three combinated IC tests (PRV, TGEV and PEDV) for the on-site, rapid screening of porcine diarrhea cases.
Keyword
Immunochromatography stripMonoclonal antibodyPorcine diarrheaPorcine rotavirusVP6 protein
ISSN
0166-0934
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.jviromet.2007.06.002
Type
Article
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
Division of Biomaterials Research > Cell Factory Research Center > 1. Journal Articles
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