Screening of the genes expressed in Pichia pastoris grown in phosphate-limited chemostat culture = 인산제한상태에서 발현되는 Pichia pastoris 유래 유전자 탐색

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Title
Screening of the genes expressed in Pichia pastoris grown in phosphate-limited chemostat culture = 인산제한상태에서 발현되는 Pichia pastoris 유래 유전자 탐색
Author(s)
Ji Yeon Hong; Jungoh Ahn; M S Park; S Y Choi; Eui Sung Choi; Joon Ki Jung; Hong-Weon Lee
Bibliographic Citation
Korean Journal of (Applied) Microbiology & Biotechnology, vol. 35, no. 4, pp. 272-277
Publication Year
2007
Abstract
The physiological responses of microorganisms to specific nutrient limitation can be regulated at the transcriptional levels. In this study, in order to develop the Pichia pastoris-derived promoter inducible by nutrient-limited condition, we constructed cDNA libraries using RT-PCR of total RNA from P. pastoris in steady-states of phosphate-limited chemostat with different dilution rates. Various genes were detected from cDNA library. Among these genes, the gene encoding putative sodium/phosphate (Na+/Pi) symporter (NPS), high affinity transporter of phosphate, was detected. It was observed that expression of NPS increased in a manner specific to phosphate-limited condition through Northern blot. Therefore, it is thought that the promoter from NPS gene may have the potential as auto-inducible promoter by phosphate-limited culture condition without inducer.
Keyword
phosphate limitationpichia pastorissodium-phosphate (Na+/Pi) symporter (NPS)
ISSN
0257-2389
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
Division of Bio Technology Innovation > 1. Journal Articles
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