Assessment of the persistence of DNA in decomposing leaves of CMVP0-CP transgenic chili pepper in the field conditions = 포장조건에서 CMVP0-CP형질전환 고추 도입유전자의 지속성 조사

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Title
Assessment of the persistence of DNA in decomposing leaves of CMVP0-CP transgenic chili pepper in the field conditions = 포장조건에서 CMVP0-CP형질전환 고추 도입유전자의 지속성 조사
Author(s)
BumKyu Lee; Chang-Gi Kim; Ji Young Park; Kee Woong Park; Hoonbok Yi; C H Harn; Hwan Mook Kim
Bibliographic Citation
Korean Journal of Environmental Agriculture, vol. 26, no. 4, pp. 319-324
Publication Year
2007
Abstract
This study was conducted to evaluate the persistence of DNA in the transgenic chili pepper resistant to cucumber mosaic virus (CMV) in the field condition. We analyzed the persistence of genes in the leaf samples obtained from two field conditions, below and above soil. Transgenic and non-transgenic leaf tissues were buried in the soil at a depth of 10 cm or placed on the soil surface. Qualitative and quantitative PCR analysis showed that the amount of transferred genes from the transgenic peppers below and above soil was dropped to 28.3-42.7% one month after buried and it was rapidly reduced to 0.9-3.3% after two months. The transgenes were not detected three to four month after buried. In addition, DNA of the leaves placed below soil decomposed about 8%more than those on soil surface. The gene transfer from decomposing leaves of the transgenic pepper to soil was investigated by PCR analysis with the soil attached to the samples. The PCR result indicated that the gene transfer from the transgenic pepper to soil was not occurred.
Keyword
DNA persistencechili peppergene transfersoiltransgenic plant
ISSN
I000-0139
Publisher
South Korea
DOI
http://dx.doi.org/10.5338/KJEA.2007.26.4.319
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > Bio-Evaluation Center > 1. Journal Articles
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