Biochemical characterization and sequence analysis of a xylanase produced by an exo-symbiotic bacterium of Gryllotalpa orientalis, Cellulosimicrobium sp. HY-12
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- Title
- Biochemical characterization and sequence analysis of a xylanase produced by an exo-symbiotic bacterium of Gryllotalpa orientalis, Cellulosimicrobium sp. HY-12
- Author(s)
- Hyun Woo Oh; Sun Yeon Heo; Do Young Kim; Doo Sang Park; Kyung Sook Bae; Ho Yong Park
- Bibliographic Citation
- Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, vol. 93, no. 4, pp. 437-442
- Publication Year
- 2008
- Abstract
- An exo-symbiotic bacterium capable of hydrolyzing xylan was isolated from the gut of the mole cricket, Gryllotalpa orientalis, and identified as Cellulosimicrobium sp. HY-12. The xylanase (XylACspHY-12) of this organism bound tightly to both DEAE and mono Q resins, and its molecular mass (Mr) was about 39.0 kDa. The highest xylanase activity was observed at pH 6.0 and 60°C. The enzyme was greatly suppressed by Ca2+, Cu2+, Co2+, and Fe2+ ions but not by Mg2+ and Mn2+. Although XylACspHY-12 was capable of hydrolyzing various types of xylosic compounds, it could not decompose carboxymethyl cellulose or xylobiose. The xylACspHY-12 gene consisted of an 1,188 bp open reading frame that encoded a polypeptide of 395 amino acids with a deduced molecular mass of 42,925 Da. The domain structure of XylACspHY-12 was most similar to those of the glycoside hydrolase (GH) family 10 endoxylanases. However its sequence identity with any of the enzymes in this family was below 52%. The results of this study suggest that the XylACspHY-12 is a new cellulase-free endo-β-1,4-xylanase with some properties that are distinct from those of GH family 10.
- Keyword
- Cellulosimicrobium sp. HY-12Exo-symbiotic bacteriumGlycoside hydrolase family 10Xylanase
- ISSN
- 0003-6072
- Publisher
- Springer
- DOI
- http://dx.doi.org/10.1007/s10482-007-9210-2
- Type
- Article
- Appears in Collections:
- Division of Bio Technology Innovation > Core Research Facility & Analysis Center > 1. Journal Articles
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
Division of Biomedical Research > Microbiome Convergence Research Center > 1. Journal Articles
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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