Development of biologically active compounds from edible plant sources XXII : Isolation of indoles from the roots of Brassica campestris ssp rapa and their hACAT inhibitory activity = 식용식물자원으로부터 활성물질의 탐색 XXII : 순무 뿌리로부터 인돌 화합물의 분리 및 hACAT 저해 활성

Abstract

The roots of Brassica campestris ssp rapa were extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and H2O. From the EtOAc fraction, three compounds were isolated through the repeated silica gel and octadecyl silica gel (ODS) column chromatography. From the results of spectroscopic data including NMR and MS, the chemical structures of the compounds were determined as caulilexin C (1), indoleacetonitrile (2) and arvelexin (3). The arvelexin (3) has been isolated from this plant for the first time. Compounds 1, 2 and 3 showed inhibitory activity on human Acyl CoA: cholesterol transferase I (hACATI) by 54.6 +/- 6.0%, 69.2 +/- 4.7% and 68.6 +/- 3.7%, and on human Acyl CoA: cholesterol transferase 2 (hACAT2) by 4.8 +/- 13.4%, 45.6 +/- 4.8%, and 39.5 +/- 4.3%, respectively, at 100 mu g/ml.