Agrobacterium-mediated genetic transformation of radish (Raphanus sativus L.)

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Title
Agrobacterium-mediated genetic transformation of radish (Raphanus sativus L.)
Author(s)
M A Cho; Sung Ran Min; S M Ko; Jang Ryol Liu; P S Choi
Bibliographic Citation
Plant Biotechnology, vol. 25, no. 2, pp. 205-208
Publication Year
2008
Abstract
In order to generate transgenic radish (Raphanus sativus L., cv. Jin Ju Dae Pyong), hypocotyl explants were cultured on Murashige and Skoog medium containing 4mg l-1 AgNO3, 5mg l-1 acetosyringone, 4mg l-1 6-benzyladenine, and 3mg l-1 α-naphthaleneacetic acid in addition to either 10 mg l-1 hygromycin or 100 mg l-1 paromomycin after cocultivation with disarmed Agrobacterium tumefaciens harboring a plant expression binary vector. Explants co-cultivated with A. tumefaciens GV3101 harboring pCAMBIA1301 and A. tumefaciens EHA101 harboring pPTN290 produced putative transgenic adventitious shoots at frequencies of 0.26% and 0.18%, respectively. Northern blot analysis revealed the gus gene transcript was detected in 8 regenerated plants which confirmed their genetic transformation. The transgenic plants were grown to maturity after vernalization in a greenhouse and appeared morphologically normal. Progeny analysis of independent transgenic plants demonstrated that the gus gene was transmitted in a Mendelian pattern in 3 lines, indicating a single copied gene was incorporated into the genome.
Keyword
AgrobacteriumHygromycinParomomycinTransgenic radishβ-glucuronidase (GUS)
ISSN
1342-4580
Publisher
Japanese Soc Plant Cell & Molecular Biology
DOI
http://dx.doi.org/10.5511/plantbiotechnology.25.205
Type
Article
Appears in Collections:
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
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