Biosynthesis of rubradirin as an ansamycin antibiotic from Streptomyces achromogenes var. rubradiris NRRL3061

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Title
Biosynthesis of rubradirin as an ansamycin antibiotic from Streptomyces achromogenes var. rubradiris NRRL3061
Author(s)
C G Kim; J Lamichhane; K I Song; V D Nguyen; D H Kim; T S Jeong; S H Kang; K W Kim; J Maharjan; Young-Soo Hong; J S Kang; J C Yoo; Jung Joon Lee; T J Oh; K Liou; J K Sohng
Bibliographic Citation
Archives of Microbiology, vol. 189, no. 5, pp. 463-473
Publication Year
2008
Abstract
The four overlapping cosmids from the rubradirin producer, Streptomyces achromogenes var rubradiris NRRL 3061, have 58 ORFs within a 105.6 kb fragment. These ORFs harbored essential genes responsible for the formation and attachment of four distinct moieties, along with the genes associated with regulatory, resistance, and transport functions. The PKS (rubA) and glycosyltransferase (rubG2) genes were disrupted in order to demonstrate a complete elimination of rubradirin production. The rubradirin biosynthetic pathway was proposed based on the putative functions of the gene products, the functional identification of sugar genes, and the mutant strains.
Keyword
AnsamycinBiosynthesisPolyketide synthaseRubradirinRubranitroseStreptomyces
ISSN
0003-9276
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s00203-007-0337-3
Type
Article
Appears in Collections:
Ochang Branch Institute > Anticancer Agent Research Center > 1. Journal Articles
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