Role of STAT3 as a negative regulator in Mac2-binding protein expression = Mac-2 결합단백질 발현에서의 STAT3의 역할

Cited 2 time in scopus
Metadata Downloads
Title
Role of STAT3 as a negative regulator in Mac2-binding protein expression = Mac-2 결합단백질 발현에서의 STAT3의 역할
Author(s)
Yuk Pheel Park; Jong-Tae Kim; Y Yang; J S Lim; D Y Yoon; J W Kim; Hee Gu Lee
Bibliographic Citation
Korean Journal of Laboratory Medicine, vol. 28, no. 3, pp. 230-238
Publication Year
2008
Abstract
BACKGROUND: Mac-2 binding protein (Mac-2BP) is a secreted glycoprotein from the culture fluid of several human cancer cells, especially breast, lung, and gastric cells. Mac-2BP plays a role in immune response and cell adhesion activity in patients with various cancer and infectious diseases. In this study, we attempted to identify the regulators of Mac-2BP expression at the transcriptional level. METHODS: To determine the effect of epidermal growth factor (EGF) to Mac-2BP expression in gastric cancers, we constructed the different lengths of Mac-2BP promoter plasmids and measured the promoter activity and Mac-2BP expression. In addition to investigating the role of signal transducer and activator of transcription3 (STAT3) or human telomerase reverse transcriptase (hTERT) as a regulator of Mac-2BP, we transfected the small interfering RNA (siRNA) specific for STAT3 or hTERT, and Mac-2BP level was observed by a quantitative ELISA. RESULTS: EGF treatment could suppress the Mac-2BP transcription in HEK293 or gastric cancer cell lines (SNU-638 or AGS). In 5'-deleted promoter experiment, pGL3-Mac Pro-2377 transfected cells showed a decreased luciferase activity compared to pGL3-Mac Pro-2277. We also identified that (-2,366/-2,356) on Mac-2BP promoter is a putative STAT3 binding site and suppression of STAT3 with STAT3 specific siRNA increased the Mac-2BP level, suggesting the role of STAT3 as a negative regulator, in contrast to hTERT, which is known as a positive regulator. CONCLUSIONS: EGF signal is critical for the Mac-2BP expression, and more importantly, STAT3 could work as a negative regulator, while hTERT as a positive regulator in Mac-2BP transcription.
Keyword
EGFhTERTMac-2BPSTAT3
ISSN
I000-0170
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.3343/kjlm.2008.28.3.230
Type
Article
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.