Transcriptional targeting of gene expression in breast cancer by the promoters of protein regulator of cytokinesis 1 and ribonuclease reductase 2 = 싸이토키네시스1 과 리보뉴클레아제 리덕테아제 2의 프로모터에 의한 유방암에서의 유전자 발현의 전사 표적

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Title
Transcriptional targeting of gene expression in breast cancer by the promoters of protein regulator of cytokinesis 1 and ribonuclease reductase 2 = 싸이토키네시스1 과 리보뉴클레아제 리덕테아제 2의 프로모터에 의한 유방암에서의 유전자 발현의 전사 표적
Author(s)
H J Yun; Y H Cho; Y Moon; Young Woo Park; H K Yoon; Y J Kim; S H Cho; Y I Lee; B S Kang; W J Kim; K Park; W Seol
Bibliographic Citation
Experimental and Molecular Medicine, vol. 40, no. 3, pp. 345-353
Publication Year
2008
Abstract
For cancer gene therapy, cancer-specific overexpression of a therapeutic gene is required to reduce side effects derived from expression of the gene in normal cells. To develop such an expression vector, we searched for genes over-expressed and/or specifically expressed in cancer cells using bioinformatics and have selected genes coding for protein regulator of cytokinesis 1 (PRC1) and ribonuclease reductase 2 (RRM2) as candidates. Their cancer-specific expressions were confirmed in both breast cancer cell lines and patient tissues. We compared each promoter's cancer-specific activity in the breast normal and cancer cell lines using the luciferase gene as a reporter and confirmed cancer-specific expression of both PRC1 and RRM2 promoters. To test activities of these promoters in viral vectors, the promoters were also cloned into an adeno-associated viral (AAV) vector containing green fluorescence protein (GFP) as the reporter. The GFP expression levels by these promoters were various depending on cell lines tested and, in MDA-MB-231 cells, GFP activities derived from the PRC1 and RRM2 promoters were as strong as that from the cytomegalovirus (CMV) promoter. Our result showed that a vector containing the PRC1 or RRM2 promoter could be used for breast cancer specific overexpression in gene therapy.
Keyword
breast neoplasmsgene therapyPRC1 protein, humanribonucleotide reductase M2
ISSN
I000-0028
Publisher
Springer-Nature Pub Group
DOI
http://dx.doi.org/10.3858/emm.2008.40.3.345
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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