Identification of small molecules inducing apoptosis by cell-based assay using fission yeast deletion mutants

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Title
Identification of small molecules inducing apoptosis by cell-based assay using fission yeast deletion mutants
Author(s)
Kyung Sook Chung; Nam-Hui Yim; S H Lee; Shin Jung Choi; K S Heo; Kwang Lae Hoe; Dong Uk Kim; S Goehle; H B Kim; K B Song; Hyang Sook Yoo; K H Bae; J Simon; Mi Sun Won
Bibliographic Citation
Investigational New Drugs, vol. 26, no. 4, pp. 299-307
Publication Year
2008
Abstract
The cell-based assay using yeast deletion mutants has been recognized as an efficient analysis to discover therapeutic compounds and reveal their mode of action. In this study, S. pombe deletion mutants-based HTS screening was carried out to identify potential anti-cancer agents. The NCI chemical library of 5700 compounds was screened using kit strains, which consisted of S. pombe mutants harboring deletions in genes involved in DNA repair and mitotic control. During the screening, we identified 40 compounds conferring growth inhibition of S. pombe. Their anti-tumorigenic properties were examined by phenotypic effect on S. pombe, flow cytometry and apoptosis analysis of human cancer. Here, we report hit compounds inducing apoptosis for development of anti-cancer agents suggesting that S. pombe deletion mutants are useful in identifying potential anti-cancer agents in human cancer therapeutics.
Keyword
Anti-cancer compoundApoptosisFission yeast
ISSN
0167-6997
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s10637-007-9100-5
Type
Article
Appears in Collections:
Division of Research on National Challenges > 1. Journal Articles
Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
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