Development of microbial genome-probing microarrays using digital multiple displacement amplification of uncultivated microbial single cells = 미배양 단일 세포의 디지털 다중증폭법을 통한 미배양 미생물 모니터링용 GPM 개발

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Title
Development of microbial genome-probing microarrays using digital multiple displacement amplification of uncultivated microbial single cells = 미배양 단일 세포의 디지털 다중증폭법을 통한 미배양 미생물 모니터링용 GPM 개발
Author(s)
Ho Won Chang; Y Sung; Kyoung-Ho Kim; Young Do Nam; Seong Woon Roh; Min-Su Kim; C O Jeon; Jin-Woo Bae
Bibliographic Citation
Environmental Science & Technology, vol. 42, no. 16, pp. 6058-6064
Publication Year
2008
Abstract
A crucial problem in the use of previously developed genome-probing microarrays (GPM) has been the inability to use uncultivated bacterial genomes to take advantage of the high sensitivity and specificity of GPM in microbial detection and monitoring. We show here a method, digital multiple displacement amplification (MDA), to amplify and analyze various genomes obtained from single uncultivated bacterial cells. We used 15 genomes from key microbes involved in dichloromethane (DCM)-dechlorinating enrichment as microarray probes to uncover the bacterial population dynamics of samples without PCR amplification. Genomic DNA amplified from single cells originating from uncultured bacteria with 80.3-99.4% similarity to 16S rRNA genes of cultivated bacteria. The digital MDA-GPM method successfully monitored the dynamics of DCM-dechlorinating communities from different phases of enrichment status. Without a priori knowledge of microbial diversity, the digital MDA-GPM method could be designed to monitor most microbial populations in a given environmental sample.
ISSN
0013-936X
Publisher
Amer Chem Soc
DOI
http://dx.doi.org/10.1021/es8006029
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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