Identification of novel retromer complexes in the mouse testis = 마우스 정소에서 정소특이적인 리트로마 복합체 동정

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Title
Identification of novel retromer complexes in the mouse testis = 마우스 정소에서 정소특이적인 리트로마 복합체 동정
Author(s)
Ekyune Kim; Jae Woong Lee; Dong Chul Baek; Sang Rae Lee; Myeong Su Kim; Sang-Hyun Kim; K Imakawa; Kyu Tae Chang
Bibliographic Citation
Biochemical and Biophysical Research Communications, vol. 375, no. 1, pp. 16-21
Publication Year
2008
Abstract
A family of vacuolar protein sorting (Vps) proteins, which are components of mammalian retromer complex, has been studied in the mouse. Vps26a is known as a retromer component that plays an important role in embryonic development: however, its cell-type expression and precise role remain to be elucidated. In this study, we identified a new isoform of Vps26a, called Vps26aT, which was expressed specifically in the mouse testis. Diverse expression patterns of Vps26 variants in mouse tissues were determined by Western blot and RT-PCR analyses, and the direct interaction of Vps26aT with Vps35 was also demonstrated by immunoprecipitation and pull-down assay using antibodies raised against each Vps component. Our results revealed that the retromer complex could be formed from different Vps26 isoforms in a tissue-specific manner, resulting in more than two types of the retromer complex, including the Vps26a-Vps29-Vps35, Vps26aT-Vps29-Vps35, and Vps26b-Vps29-Vps35 complexes in mouse tissues.
Keyword
Embryonic developmentEndosomeRetromer complexSpermatogenesisVacuolar protein sorting
ISSN
0006-291X
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.bbrc.2008.07.067
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of National Bio-Infrastructure > National Primate Research Center > 1. Journal Articles
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