Transgenic tomatoes expressing human beta-amyloid for use as a vaccine against Alzheimer's disease

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Title
Transgenic tomatoes expressing human beta-amyloid for use as a vaccine against Alzheimer's disease
Author(s)
Jung Won Youm; Jae Heung Jeon; H Kim; Y H Kim; K Ko; Hyouk Joung; Hyun Soon Kim
Bibliographic Citation
Biotechnology Letters, vol. 30, no. 10, pp. 1839-1845
Publication Year
2008
Abstract
Human β-amyloid (Aβ) is believed to be one of the main components of Alzheimer's disease, so reduction of Aβ is considered a key therapeutic target. Using Agrobacterium-mediated nuclear transformation, we generated transgenic tomatoes for Aβ with tandem repeats. Integration of the human Aβ gene into the tomato genome and its transcription were detected by PCR and Northern blot, respectively. Expression of the Aβ protein was confirmed by western blot and ELISA, and then the transgenic tomato line expressing the highest protein level was selected for vaccination. Mice immunized orally with total soluble extracts from the transgenic tomato plants elicited an immune response after receiving a booster. The results indicate that tomato plants may provide a useful system for the production of human Aβ antigen.
Keyword
Alzheimer's diseaseedible vaccineimmunogenicitytomato-derived beta amyloid
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s10529-008-9759-5
Type
Article
Appears in Collections:
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
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