Microarray study of genes differentially modulated in response to nitric oxide in macrophages

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Title
Microarray study of genes differentially modulated in response to nitric oxide in macrophages
Author(s)
X Nan; O Maeng; H Shin; H J An; Young Il Yeom; H Lee; S G Paik
Bibliographic Citation
Animal Cells and Systems, vol. 12, no. 1, pp. 15-21
Publication Year
2008
Abstract
Nitric oxide (NO) has been known to play important roles in numerous physiologic processes including neurotransmission, vasorelaxation, and cellular apoptosis. Using a mouse cDNA gene chip, we examined expression patterns and time course of NO-dependent genes in mouse macrophage RAW264.7 cells. Genes shown to be upregulated more than two fold or at least at two serial time points were further selected and validated by RT-PCR. Finally, 81 selected genes were classified by function as signaling, apoptosis, inflammation, transcription, translation, ionic homeostasis and metabolism. Among those, genes related with signaling, apoptosis and inflammation, such as guanylate cyclase 1, soluble, alpha3 (Gucy1a3); protein kinase C, alpha (Pkcα); lymphocyte protein tyrosine kinase (Lck); BCL2/adenovirus E1B 19 kDa-interacting protein (Bnip3); apoptotic protease activating factor 1 (Apaf1); Xlinked inhibitor of apoptosis (Xiap); cyclin G1 (Ccng1); chemokine (C-C motif) ligand 4 (Ccl4); B cell translocation gene 2, anti-proliferative (Btg2); lysozyme 2 (Lyz2); secreted phosphoprotein 1 (Spp1); heme oxygenase (decycling) 1 (Hmox1); CD14 antigen (Cd14); and granulin (Grn) may play important roles in NO-dependent responses in murine macrophages.
Keyword
nitric oxidemicroarraymouse macrophage RAW264.7 cellsapoptosis
ISSN
1976-8354
Publisher
T&F (Taylor & Francis)
DOI
http://dx.doi.org/10.1080/19768354.2008.9647149
Type
Article
Appears in Collections:
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
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