Epigallocatechin gallate stimulates glucose uptake through the phosphatidylinositol 3-kinase-mediated pathway in L6 rat skeletal muscle cells = Epigallocatechin gallate는 L6마우스 골근세포 phosphatidylinositol 3-kinase-mediated pathway통해 포도당흡수 촉진

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Title
Epigallocatechin gallate stimulates glucose uptake through the phosphatidylinositol 3-kinase-mediated pathway in L6 rat skeletal muscle cells = Epigallocatechin gallate는 L6마우스 골근세포 phosphatidylinositol 3-kinase-mediated pathway통해 포도당흡수 촉진
Author(s)
K H Jung; H S Choi; D H Kim; M Y Han; U J Chang; S V Yim; B C Song; Chul Ho Kim; S A Kang
Bibliographic Citation
Journal of Medicinal Food, vol. 11, no. 3, pp. 429-434
Publication Year
2008
Abstract
The effect of epigallocatechin gallate (EGCG) on glucose uptake was studied in L6 rat skeletal muscle cells. Glucose uptake assay revealed that EGCG increased glucose uptake >70% compared to control. EGCG-stimulated glucose uptake was blocked by LY294002, an inhibitor of phosphatidylinositol (PI) 3-kinase, which is a major regulatory molecule in glucose uptake pathways. However, AMP-activated protein kinase (AMPK), which is another crucial mediator in independent glucose uptake pathways, did not inhibit EGCG-stimulated glucose uptake by SB203585, a specific inhibitor of the AMPK downstream mediator, p38 mitogen-activated protein kinase (MAPK). We also found that EGCG increased the phosphorylation level of protein kinase B and PI 3-kinase activity, when assessed by PI 3-kinase assay, whereas no increase in the phosphorylation level of AMPK and p38 MAPK was observed. Taken together, these results suggest that EGCG might stimulate glucose uptake, not AMPK-mediated but PI 3-kinase-mediated, in skeletal muscle cells, thereby contributing to glucose homeostasis.
Keyword
AMP-activated protein kinaseEpigallocatechin gallateGlucose uptakePhosphatidylinositol 3-kinaseSkeletal muscle
ISSN
1096-620X
Publisher
Mary Ann Liebert, Inc
DOI
http://dx.doi.org/10.1089/jmf.2007.0107
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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