Molecular characterization of the DYX1C1 gene and its application as a cancer biomarker

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Molecular characterization of the DYX1C1 gene and its application as a cancer biomarker
Y J Kim; Jae Won HuhDae Soo Kim; M I Bae; J R Lee; H S Ha; K Ahn; T O Kim; G A Song; H S Kim
Bibliographic Citation
Journal of Cancer Research and Clinical Oncology, vol. 135, no. 2, pp. 265-270
Publication Year
Purpose: DYX1C1 has three alternatively spliced transcripts. Therefore, we expect that alternative transcripts of DYX1C1 are used as a biomarker to detect specific cancer. Methods: RT-PCR analysis is conducted in order to detect expression of the DYX1C1 gene and the PCR products were analyzed using the Image J program to compare the expression levels of each transcript. Results: We found one of the transcripts was directly associated with an HERV-H LTR element that could be translated into protein sequence. Four new alternative transcripts were identified by RT-PCR analysis with various human tissue samples including 10 normal and adjacent tumor tissue sets. Semi-quantitative RT-PCR analysis showed the transcriptional activity of V3 and V2 was higher in tumor than in normal tissue samples, especially in the colorectal tissue samples. Conclusion: Our results indicated that alternatively spliced transcript variants of the DYX1C1 gene could be used as cancer biomarkers to detect colorectal cancer.
alternative splicingdyslexia susceptibility 1 candidate 1 (DYX1C1)human endogenous retrovirus (HERV)long terminal repeat (LTR)
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Ochang Branch Institute > Division of National Bio-Infrastructure > National Primate Research Center > 1. Journal Articles
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