Molecular characterization of the DYX1C1 gene and its application as a cancer biomarker

Cited 14 time in scopus
Metadata Downloads
Title
Molecular characterization of the DYX1C1 gene and its application as a cancer biomarker
Author(s)
Y J Kim; Jae Won HuhDae Soo Kim; M I Bae; J R Lee; H S Ha; K Ahn; T O Kim; G A Song; H S Kim
Bibliographic Citation
Journal of Cancer Research and Clinical Oncology, vol. 135, no. 2, pp. 265-270
Publication Year
2009
Abstract
Purpose: DYX1C1 has three alternatively spliced transcripts. Therefore, we expect that alternative transcripts of DYX1C1 are used as a biomarker to detect specific cancer. Methods: RT-PCR analysis is conducted in order to detect expression of the DYX1C1 gene and the PCR products were analyzed using the Image J program to compare the expression levels of each transcript. Results: We found one of the transcripts was directly associated with an HERV-H LTR element that could be translated into protein sequence. Four new alternative transcripts were identified by RT-PCR analysis with various human tissue samples including 10 normal and adjacent tumor tissue sets. Semi-quantitative RT-PCR analysis showed the transcriptional activity of V3 and V2 was higher in tumor than in normal tissue samples, especially in the colorectal tissue samples. Conclusion: Our results indicated that alternatively spliced transcript variants of the DYX1C1 gene could be used as cancer biomarkers to detect colorectal cancer.
Keyword
alternative splicingdyslexia susceptibility 1 candidate 1 (DYX1C1)human endogenous retrovirus (HERV)long terminal repeat (LTR)
ISSN
0171-5216
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s00432-008-0445-8
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of National Bio-Infrastructure > National Primate Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.