Identification and molecular characterization of PERV gamma1 long terminal repeats = PERV gamma1 LTR의 동정과 분자적인 특성 규명

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Title
Identification and molecular characterization of PERV gamma1 long terminal repeats = PERV gamma1 LTR의 동정과 분자적인 특성 규명
Author(s)
Jae Won HuhDae Soo Kim; H S Ha; K Ahn; Kyu Tae Chang; B W Cho; H S Kim
Bibliographic Citation
Molecules and Cells, vol. 27, no. 1, pp. 119-123
Publication Year
2009
Abstract
Porcine endogenous retroviruses (PERVs) gamma1 in the pig genome have the potential to act as harmful factors in xenotransplantation (pig-to-human). Long terminal repeats (LTRs) are known to be strong promoter elements that could control the transcription activity of PERV elements and the adjacent functional genes. To investigate the transcribed PERV gamma1 LTR elements in pig tissues, bioin-formatic and experimental approaches were conducted. Using RT-PCR amplification and sequencing approaches, 69 different transcribed LTR elements were identified. And 69 LTR elements could be divided into six groups (15 subgroups) by internal variation including tandem repeated sequences, insertion and deletion (INDEL). Remarkably, all internal variations were indentified in U3 region of LTR elements. Taken together, the identification and characterization of various PERV LTR transcripts allow us to extend our knowledge of PERV and its transcriptional study.
Keyword
BioinformaticsLTR elementPERVPigXenotransplantation
ISSN
1016-8478
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.1007/s10059-009-0013-3
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of National Bio-Infrastructure > National Primate Research Center > 1. Journal Articles
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